Вставьте данные из отчета NanoDrop (весь блок с заголовками) для автоматического расчета разведений и анализа чистоты.
| ID | Conc. | 260/280 | 260/230 | V1 (сток) | V3 (H2O) |
|---|
Примечание: 🟢 — Норма | 🟡 — Риск (РНК/соли/деградация ДНК) | 🔴 — Грязный образец (белок/фенол)
- Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
- Olson, N. D., & Morrow, J. B. (2012). Standardization of solute concentration by UV-vis spectrophotometry. Analytical Methods, 4(2), 383-389.
- Manchester, K. L. (1996). Use of UV methods for measurement of protein and nucleic acid concentrations. Biotechniques, 20(6), 968-970.
- William W. Wilfinger, Karol Mackey, and Piotr Chomczynski (1997). Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity. BioTechniques, 22:3, 474-481.
- Thermo Scientific (2010). Assessment of Nucleic Acid Purity: 260/280 and 260/230 Ratios. Technical Bulletin NanoDrop, T042.
- Koetsier, G., & Cantor, E. (2019). A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers. NEB Expressions, Issue I.
Sleptcov A. Online Laboratory Calculators for Molecular Biology and Chemistry: A Collection of Tools (v1.0). Zenodo. 2026. DOI: 10.5281/zenodo.19404378. (Accessed: ).